DIFFERENTIAL REGULATION OF MYOD AND MYOGENIN MESSENGER-RNA LEVELS BY NERVE INDUCED MUSCLE-ACTIVITY

被引：117

作者：

WITZEMANN, V

SAKMANN, B

机构：

[1] Max-Planck-Institut für medizinische Forschung, Abteilung Zellphysiologie, Heidelberg

来源：

FEBS LETTERS | 1991年 / 282卷 / 02期

关键词：

MYOD; MYOGENIN; MESSENGER RNA LEVEL; MUSCLE ACTIVITY; RAT;

D O I：

10.1016/0014-5793(91)80490-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The levels of mRNAs coding for the myogenic factors MyoD and myogenin were measured during synapase formation in developing muscle and in adult muscle, after denervation and reinnervation and after muscle paralysis induced by blocking of neuromuscular transmission by neurotoxins known to alter the density and localization of synaptic proteins such as the acetylcholine receptor (AChR). The mRNA levels of both factors depend on usage of the neuromuscular synapses, but they change to different extents. Myogenin mRNA levels decrease drastically with innervation and increase strongly following blocking of transmission whereas the level of MyoD mRNA showed only a small decrease in response to innervation, denervation or muscle paralysis by neurotoxins. Neither mRNA showed a synapse-related cellular distribution. The results suggest that nerve-induced electrical muscle activity determines the cellular ratio of MyoD and myogenin mRNAs in adult muscle.

引用

页码：259 / 264

页数：6

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