CORRELATION BETWEEN NMR SPECTRAL PARAMETERS OF NUCLEOSIDES AND ITS IMPLICATION TO THE CONFORMATION ABOUT THE GLYCOSYL BOND

Analyses of high resolution proton and carbon NMR spectra of a series of guanine nucleosides in DMSO have revealed a near linear correlation between the chemical shift of the H2, atom of the sugar moiety and the vicinal coupling constant 3JC4-H1. This unexpected result provides evidence that the variations in the glycosyl torsion angle between nucleosides in solution are less that those which have previously been reported in crystals and it is an experimental basis for analyzing the syn and anti populations from chemical shift and coupling constant data. © 1991 Academic Press, Inc.