ISOLATION OF A METHANOL DEHYDROGENASE WITH A FUNCTIONAL COUPLING TO CYTOCHROME-C

A methanol dehydrogenase containing cytochrome c which was reduced on additon of methanol was isolated from Hyphomicrobium X. Metal-chelators or O2 blocked this electron transport. O2 changed the coupling irreversibly, transforming the enzyme in the complex into the already known, NH4+-requiring enzyme form. This classifical methanol dehydrogenase differs in several respects from the more natural enzyme reported here.