DETECTION OF MYCOPLASMA-HYOPNEUMONIAE BY USING RIBOSOMAL-RNA-OLIGODEOXYNUCLEOTIDE HYBRIDIZATION

被引：7

作者：

FUTO, S ^{[1
]}

SETO, Y ^{[1
]}

MITSUSE, S ^{[1
]}

MORI, Y ^{[1
]}

机构：

[1] NATL INST ANIM HLTH,TSUKUBA,IBARAKI,JAPAN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 06期

关键词：

D O I：

10.1128/JCM.30.6.1509-1513.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A system that uses rRNA-oligodeoxynucleotide hybridization was developed for the detection of Mycoplasma hyopneumoniae. Synthetic oligonucleotide MHP1 was hybridized specifically with M. hyopneumoniae. Furthermore, the detection of M. hyopneumoniae in clinical samples, such as bronchoalveolar lavage fluid and lung lesions from experimentally infected pigs, was evaluated by this assay. The evidence obtained from the assay indicated that the system can be used to efficiently diagnose mycoplasmal pneumonia of swine. Additionally, a nonradioisotopic system with chemiluminescence detection was tested. This system was 10-fold less sensitive than a test that used radioisotopes.

引用

页码：1509 / 1513

页数：5

共 26 条

[1] A GENERAL-METHOD TO GENERATE DNA PROBES FOR MICROORGANISMS [J].