PLANT-REGENERATION THROUGH SOMATIC EMBRYOGENESIS IN PEANUT (ARACHIS-HYPOGAEA L)

Somatic embryos were induced from immature cotyledons and immature embryonal axis of Arachis hypogaea L. on L-6 basal medium supplemented with NAA, picloram or 2,4-D at 5-50 mg l-1. Immature embryonal axis produced a higher number of somatic embryos in comparison with immature cotyledons. The highest number of responding cultures was produced on medium supplemented with NAA (50 mg l-1), while the highest average number of somatic embryos per culture was produced on medium with 2,4-D (10 or 20 mg l-1) and picloram (30 mg l-1) from cotyledons. The somatic embryos developed into plants on basal medium supplemented with activated charcoal and about 100 plants were successfully transferred to the field.