TRANSFECTION OF FOLATE-POLYLYSINE DNA COMPLEXES - EVIDENCE FOR LYSOSOMAL DELIVERY

被引：112

作者：

MISLICK, KA

BALDESCHWIELER, JD

KAYYEM, JF

MEADE, TJ

机构：

[1] CALTECH,DIV CHEM & CHEM ENGN,PASADENA,CA 91125

[2] CALTECH,DIV BIOL,PASADENA,CA 91125

[3] CALTECH,BECKMAN INST,PASADENA,CA 91125

来源：

BIOCONJUGATE CHEMISTRY | 1995年 / 6卷 / 05期

关键词：

D O I：

10.1021/bc00035a002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We are utilizing the folate receptor for the intracellular delivery of DNA. In this study, a folate-poly-L-lysine (FPLL) conjugate was synthesized and equilibrated with plasmid DNA encoding the firefly luciferase gene. The FPLL-DNA complexes were added to KB cells treated with chloroquine. Luciferase activity of cells incubated with FPLL-DNA was 6-fold higher than of cells exposed to poly-L-lysine (PLL)-DNA. The addition of free folic acid competitively inhibited the enhancement of gene expression. Removal of chloroquine from the media significantly inhibited transfection efficiency of FPLL-DNA complexes. We conclude that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and Likely follow a lysosomal pathway into the cytoplasm.

引用

页码：512 / 515

页数：4

共 23 条

[1] POTOCYTOSIS - SEQUESTRATION AND TRANSPORT OF SMALL MOLECULES BY CAVEOLAE [J].