INTERPHASE CYTOGENETIC ANALYSIS OF ERBB2 AND TOPOII-ALPHA COAMPLIFICATION IN INVASIVE BREAST-CANCER AND POLYSOMY OF CHROMOSOME-17 IN DUCTAL CARCINOMA IN-SITU

被引:49
作者
MURPHY, DS
MCHARDY, P
COUTTS, J
MALLON, EA
GEORGE, WD
KAYE, SB
BROWN, R
KEITH, WN
机构
[1] UNIV GLASGOW,BEATSON LABS,CRC,DEPT MED ONCOL,GLASGOW G61 1BD,LANARK,SCOTLAND
[2] UNIV GLASGOW,DEPT SURG,GLASGOW G61 1BD,LANARK,SCOTLAND
[3] UNIV GLASGOW,DEPT PATHOL,GLASGOW G61 1BD,LANARK,SCOTLAND
关键词
D O I
10.1002/ijc.2910640106
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Breast cancer is a genetically complex disease. Fluorescence in situ hybridisation can be used to analyse the genetics of breast-cancer progression by interphase cytogenetics. We have analysed the histological distribution of erbB2 and topoll alpha co-amplification in paraffin sections of invasive breast cancer and show that the co-amplified loci share the same histological distribution in the tumour and have a similar nuclear distribution within individual nuclei. Regions of the tumours without amplification are easily recognized and tumours with erbB2 and topoll alpha co-amplification can be distinguished from those with erbB2 amplification alone. In addition, FISH was used to show polysomy of chromosome 17 in non-invasive ductal carcinoma in situ of the breast and erbB2 amplification in both the invasive and non-invasive components of a breast cancer biopsy. This report of an interphase cytogenetic analysis of non-invasive breast carcinoma in situ demonstrates the usefulness of FISH for the genetic study of breast cancer progression. (C) 1995 Wiley-Liss, Inc.
引用
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页码:18 / 26
页数:9
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