CHLORAMPHENICOL DAMAGES BACTERIAL DNA

被引：15

作者：

JACKSON, SF ^{[1
]}

WENTZELL, BR ^{[1
]}

MCCALLA, DR ^{[1
]}

FREEMAN, KB ^{[1
]}

机构：

[1] MCMASTER UNIV, DEPT BIOCHEM, HAMILTON L8S 4J9, ONTARIO, CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1977年 / 78卷 / 01期

关键词：

D O I：

10.1016/0006-291X(77)91233-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

L(+)-threo-chloramphenicol [which is potentially mutagenic for humans] induces reversion of His- Salmonella typhimurium strains TA100 and TA1535 in the conventional Ames'' assay without microsomal activation. Any mutagenicity of D(-)-threo-chloramphenicol was masked by toxicity. Similarly, a sensitive fluctuation test showed mutagenesis with L(+)-threo-chloramphenicol at concentrations of 0.5 .mu.M and above but the D(-) isomer proved to be toxic even at these low levels. The L(+) isomer caused single strand breaks in the DNA of Escherichia coli B/r and S. typhimurium strains TA1535, TA100 and TA1976. The D(-) isomer caused breaks in E. coli B/r and S. typhimurium TA1976 although it was less effective and it did not produce DNA breaks in TA1535 or TA100.

引用

页码：151 / 157

页数：7

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