ELISA FOR IDENTIFICATION OF BLOOD MEAL SOURCE IN BLACK FLIES (DIPTERA, SIMULIIDAE)

被引：23

作者：

HUNTER, FF

BAYLY, R

机构：

[1] QUEENS UNIV,DEPT BIOL,KINGSTON K7L 3N6,ONTARIO,CANADA

[2] QUEENS UNIV,DEPT MICROBIOL & IMMUNOL,KINGSTON K7L 3N6,ONTARIO,CANADA

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 1991年 / 28卷 / 04期

关键词：

INSECTA; SIMULIIDAE; BLOOD MEAL; ELISA;

D O I：

10.1093/jmedent/28.4.527

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A modified ELISA, in which a biotinylated second antibody and a streptavidinbiotinylated peroxidase complex are used, has been developed to identify the blood meal sources of black flies. The modified assay is sensitive enough to identify correctly 100% of blood meals at 73 h postingestion and 80% of blood meals at 116 h postingestion in black flies held in the field at ambient temperatures.

引用

页码：527 / 532

页数：6

共 15 条

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