GAS-CHROMATOGRAPHY MASS-SPECTROMETRY METHODS FOR ANALYSIS OF 2-HYDROXYLATED AND 3-HYDROXYLATED FATTY-ACIDS - APPLICATION FOR ENDOTOXIN MEASUREMENT

被引：59

作者：

MIELNICZUK, Z

MIELNICZUK, E

LARSSON, L

机构：

[1] UNIV LUND, DEPT MED MICROBIOL, SOLVEGATAN 23, S-22362 LUND, SWEDEN

[2] CHILD HLTH CTR, WARSZAWA MIEDZYLESIE, POLAND

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1993年 / 17卷 / 02期

关键词：

ENDOTOXIN; DUST; GCMS; HYDROXY ACID; ENVIRONMENT;

D O I：

10.1016/0167-7012(93)90002-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gas chromatography-mass Spectrometry (GC-MS) methods for analysis of 2- and 3-hydroxy fatty acids were evaluated. These acids were analysed as methyl-trimethylsilyl or methyl-pentafluorobenzoyl derivatives in the electron impact and chemical ionization-negative ion (NICI) modes, respectively. NICI spectra were strongly influenced by the temperature of the ion source. Both 2- and 3-hydroxylated acids with 10 18 carbon atoms were detected in organic dust collected from a ventilation duct; differential ion monitoring was used for their separation. The described GC-MS methods can be applied for determination of air-borne endotoxins.

引用

页码：91 / 102

页数：12

共 17 条

[1]

[Anonymous], 1988, MICROBIAL LIPIDS

[2]

ASSELINEAU J, 1991, PROGR CHEM ORG NAT P, V56, P1

[3] ACUTE BRONCHOCONSTRICTION INDUCED BY COTTON DUST - DOSE-RELATED RESPONSES TO ENDOTOXIN AND OTHER DUST FACTORS [J].