DOES CRUCIFORM DNA PROVIDE A RECOGNITION SIGNAL FOR DNA-TOPOISOMERASE-II

被引：14

作者：

POGNAN, F

PAOLETTI, C

机构：

[1] Laboratoire de Biochimie-Enzymologie, URA 147, Institut Gustave-Roussy, 94805 Villejuif Cedex, rue Camille Desmoulins

来源：

BIOCHIMIE | 1992年 / 74卷 / 11期

关键词：

TOPOISOMERASE-II; CLEAVABLE COMPLEX; SUPERCOILED DNA; S1 NUCLEASE MAPPING; HAIRPIN DNA;

D O I：

10.1016/0300-9084(92)90022-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Topoisomerase II displays higher affinity for supercoiled DNA compared to the same relaxed DNA. Moreover, cruciform structures are formed in topologically constrained DNA. Here we report that, using S1 nuclease experiments on supercoiled DNA, hairpin structures are located close to numerous topoisomerase II cleavage sites on the BPV I genome. Therefore, DNA secondary structure may play a role in the recognition mechanism of DNA by topoisomerase II.

引用

页码：1019 / 1023

页数：5

共 20 条

[1] MULTIMODAL ACTION OF ANTITUMOR AGENTS ON DNA - THE ELLIPTICINE SERIES [J].

AUCLAIR, C .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 259 (01) :1-14

[2]

BAGULEY BC, 1991, ANTI-CANCER DRUG DES, V6, P1

[3]

DIMAIO D, 1991, ADV CANCER RES, V56, P133

[4] INHIBITORS OF DNA TOPOISOMERASES [J].

DRLICA, K ;

FRANCO, RJ .

BIOCHEMISTRY, 1988, 27 (07) :2253-2259

[5]

HALLIGAN BD, 1985, J BIOL CHEM, V260, P2475

[6] TOPOISOMERASE INHIBITORS INDUCE IRREVERSIBLE FRAGMENTATION OF REPLICATED DNA IN CONCANAVALIN-A STIMULATED SPLENOCYTES [J].

JAXEL, C ;

TAUDOU, G ;

PORTEMER, C ;

MIRAMBEAU, G ;

PANIJEL, J ;

DUGUET, M .

BIOCHEMISTRY, 1988, 27 (01) :95-99

[7] DNA TOPOISOMERASE POISONS AS ANTITUMOR DRUGS [J].

LIU, LF .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :351-375

[8]

Maxam A M, 1980, Methods Enzymol, V65, P499

[9] MECHANISTIC ASPECTS OF DNA TOPOISOMERASES [J].

MAXWELL, A ;

GELLERT, M .

ADVANCES IN PROTEIN CHEMISTRY, 1986, 38 :69-107

[10] HAIRPIN FORMATION WITHIN THE ENHANCER REGION OF THE HUMAN ENKEPHALIN GENE [J].

MCMURRAY, CT ;

WILSON, WD ;

DOUGLASS, JO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) :666-670

← 1 2 →