STEADY-STATE INVESTIGATION OF THE MECHANISM OF HISTIDINOL DEHYDROGENASE

被引：25

作者：

GORISCH, H

机构：

来源：

BIOCHEMICAL JOURNAL | 1979年 / 181卷 / 01期

关键词：

D O I：

10.1042/bj1810153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Initial velocities of the histidinol dehydrogenase reaction (EC 1.1.1.23) were measured as a function of the concentrations of the substrates histidinol and NAD+ and in the presence and absence of the product NADH. The data are consistent with a Bi Uni Uni Bi Ping Pong mechanism. The kinetic constants of this mechanism were determined; Km for histidinol was found to be 14 microM and for NAD+ 0.7 mV; Ki for NAD+ was 0.4 mM.

引用

页码：153 / 157

页数：5

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