ELECTROCHEMICAL APPROACH TO QUANTITATION AND CHARACTERIZATION OF METALLOTHIONEINS

被引：182

作者：

OLAFSON, RW

SIM, RG

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 100卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90239-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analysis was made of the potential of the electrochemical approach to quantitation and characterization of [bovine] metallothioneins [for biochemical and toxicological studies]. The Brdicka procedure for determination of sulfhydryl groups was shown to be of utility as a standard determination method while anodic stripping voltammetry was found to offer advantage in determination of protein-bound Zn and Cd. Cyclic voltammetry was observed to differentiate metallothioneins from other sulfhydryl- or disulfide-containing compounds, suggesting considerable potential as a characterizing method for metallothioneins.

引用

页码：343 / 351

页数：9

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