FLUORESCENCE QUENCHING IN THE VARIED PHOTOSYNTHETIC MODES OF PORTULACARIA-AFRA (L) JACQ

The kinetics of chlorophyll fluorescence were measured in Portulacaria afra (L.) Jacq. when the plants were functioning in either Crassulacean acid metabolism (CAM) or C3/CAM cycling (called cycling) modes, as determined by fluctuation in titratable acidity and gas exchange properties. Cycling plants showed primarily daytime CO2 uptake typical of C3 plants, but with a slight diurnal acid fluctuation, whereas CAM plants showed nocturnal CO2 uptake, daytime stomatal closure, and a large diurnal acid fluctuation. Results from fluorescence measurements indicated no significant differences in photochemical quenching between cycling and CAM plants; however, sizable differences were detected in nonphotochemical quenching (q(N)), with the largest differences being observed during the middle of the day. Cycling plants had lower q(N) than CAM plants, indicating altered photosynthetic regulation processes. This q(N) difference was believed to be related to reduced internal CO2 concentration in the CAM plants because of daytime stomatal closure and reduced deacidification rates in the late afternoon when most of the malic acid has been utilized. Experimentally, higher external CO2 given to plants in the CAM mode resulted in a decline in q(N) in comparison to that measured in plants in the cycling mode. No changes were observed in photochemical quenching when CO2 was added.