ENDOTHELIN(B) RECEPTOR ON GUINEA-PIG SMALL INTESTINAL SMOOTH-MUSCLE CELLS

被引：39

作者：

YOSHINAGA, M

CHIJIIWA, Y

MISAWA, T

HARADA, N

NAWATA, H

机构：

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 262卷 / 02期

关键词：

ENDOTHELIN-1-3; ENDOTHELIN RECEPTOR SUBTYPE; BINDING ASSAY;

D O I：

10.1152/ajpgi.1992.262.2.G308

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We investigated the binding characteristics of the endothelin (ET) receptor and the mechanism by which ET induces contraction of longitudinal smooth muscle cells of the guinea pig small intestine by using vasoactive intestinal contractor (VIC), a mouse variant of ET-2. A functional receptor for VIC was found to exist on longitudinal smooth muscle cells. These cells showed a similar binding of and contractile response to ET-1, ET-2, and ET-3. Inhibitors of both intracellular and extracellular Ca2+ movement attenuated the VIC-induced contraction of longitudinal smooth muscle cells. These results suggest that smooth muscle cells of the guinea pig small intestine express the ET(B) receptor that primarily mediates the contractile effect on smooth muscle cells. In addition, ET-induced contraction depends on intracellular as well as extracellular Ca2+.

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页码：G308 / G311

页数：4

相关论文

共 25 条

[1] CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR [J].

ARAI, H ;

HORI, S ;

ARAMORI, I ;

OHKUBO, H ;

NAKANISHI, S .

NATURE, 1990, 348 (6303) :730-732

[2] RECEPTORS ON SMOOTH-MUSCLE CELLS - CHARACTERIZATION BY CONTRACTION AND SPECIFIC ANTAGONISTS [J].

BITAR, KN ;

MAKHLOUF, GM .

AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (04) :G400-G407

[3]

CRAWFORD MLA, 1990, N-S ARCH PHARMACOL, V341, P268

[4] ENDOTHELIAN ACTIVATES THE DIHYDROPYRIDINE-SENSITIVE, VOLTAGE-DEPENDENT CA-2+ CHANNEL IN VASCULAR SMOOTH-MUSCLE [J].

GOTO, K ;

KASUYA, Y ;

MATSUKI, N ;

TAKUWA, Y ;

KURIHARA, H ;

ISHIKAWA, T ;

KIMURA, S ;

YANAGISAWA, M ;

MASAKI, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3915-3918

[5] PROPERTIES OF RAT UTERUS ENDOTHELIN RECEPTOR-SITES [J].

HAGIWARA, H ;

KOZUKA, M ;

ITO, T ;

EGUCHI, S ;

HIROSE, S .

BIOMEDICAL RESEARCH-TOKYO, 1990, 11 (02) :93-98

[6] INTERACTION OF ENDOTHELIN ISOPEPTIDES WITH VASCULAR ENDOTHELIN-1 RECEPTOR [J].

HIRATA, Y ;

YOSHIMI, H ;

TAKAGI, Y ;

KANNO, K ;

EGUCHI, S ;

MARUMO, F .

BIOMEDICAL RESEARCH-TOKYO, 1990, 11 (03) :195-198

[7] THE HUMAN ENDOTHELIN FAMILY - 3 STRUCTURALLY AND PHARMACOLOGICALLY DISTINCT ISOPEPTIDES PREDICTED BY 3 SEPARATE GENES [J].

INOUE, A ;

YANAGISAWA, M ;

KIMURA, S ;

KASUYA, Y ;

MIYAUCHI, T ;

GOTO, K ;

MASAKI, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2863-2867

[8] DIFFERENTIAL ACTIVITIES OF 2 DISTINCT ENDOTHELIN FAMILY PEPTIDES ON ILEUM AND CORONARY-ARTERY [J].

ISHIDA, N ;

TSUJIOKA, K ;

TOMOI, M ;

SAIDA, K ;

MITSUI, Y .

FEBS LETTERS, 1989, 247 (02) :337-340

[9]

KOMADA M, 1989, BIOCHEM BIOPH RES CO, V160, P1302

[10] THE ACTIVITY OF PEPTIDES OF THE ENDOTHELIN FAMILY IN VARIOUS MAMMALIAN SMOOTH-MUSCLE PREPARATIONS [J].

MAGGI, CA ;

GIULIANI, S ;

PATACCHINI, R ;

ROVERO, P ;

GIACHETTI, A ;

MELI, A .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1989, 174 (01) :23-31