STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING

被引：45

作者：

SUNGUR, S

ELCIN, YM

AKBULUT, U

机构：

[1] MIDDLE E TECH UNIV, DEPT CHEM, 06531 ANKARA, TURKEY

[2] ANKARA UNIV, DEPT CHEM, 06100 ANKARA, TURKEY

来源：

BIOMATERIALS | 1992年 / 13卷 / 11期

关键词：

IMMOBILIZATION; ENZYMES; UREASE; GELATIN;

D O I：

10.1016/0142-9612(92)90020-O

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).

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页码：795 / 800

页数：6

相关论文

共 18 条

[1] FLUOROMETRIC-DETERMINATION OF UREA BY FLOW-INJECTION ANALYSIS [J].

ABDELLATIF, MS ;

GUILBAULT, GG .

JOURNAL OF BIOTECHNOLOGY, 1990, 14 (01) :53-61

[2]

AKBULUT U, 1991, Macromolecular Reports, VA28, P239

[3] IMMOBILIZATION OF GLUCOSE-OXIDASE IN POLY(2-HYDROXYETHYL METHACRYLATE) MEMBRANES [J].

ARICA, Y ;

HASIRCI, VN .

BIOMATERIALS, 1987, 8 (06) :489-495

[4]

Bergmeyer HI., 1974, METHODS ENZYMATIC AN

[5] POLYESTER FILM STRIPS COATED WITH PHOTOGRAPHIC GELATIN CONTAINING IMMOBILIZED GLUCOSE-OXIDASE HARDENED BY CHROMIUM(III) SULFATE [J].

ELCIN, YM ;

AKBULUT, U .

BIOMATERIALS, 1992, 13 (03) :156-161

[6] MICRO-ENZYME FIELD-EFFECT TRANSISTOR SENSOR USING DIRECT COVALENT BONDING OF UREASE [J].

GARDIES, F ;

JAFFREZICRENAULT, N ;

MARTELET, C ;

PERROT, H ;

VALLETON, JM ;

ALEGRET, S .

ANALYTICA CHIMICA ACTA, 1990, 231 (02) :305-308

[7] CONVERSION OF AMMONIA OR UREA INTO ESSENTIAL AMINO-ACIDS, L-LEUCINE, L-VALINE, AND L-ISOLEUCINE USING ARTIFICIAL CELLS CONTAINING AN IMMOBILIZED MULTIENZYME SYSTEM AND DEXTRAN-NAD+ - L-LACTIC DEHYDROGENASE FOR COENZYME RECYCLING [J].

GU, KF ;

CHANG, TMS .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1990, 26 (02) :115-124

[8] PRODUCTION OF ESSENTIAL L-BRANCHED-CHAIN AMINO-ACIDS IN BIOREACTORS CONTAINING ARTIFICIAL CELLS IMMOBILIZED MULTIENZYME SYSTEMS AND DEXTRAN-NAD+ [J].

GU, KF ;

CHANG, TMS .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 36 (03) :263-269

[9]

HOLTS O, 1991, BIOSENS BIOELECTRON, V6, P101

[10]

ICHIMURA K, 1987, MAKROMOL CHEM, V188, P763