THE HUMAN RIBOSOMAL-PROTEIN S7-ENCODING GENE - ISOLATION, STRUCTURE AND LOCALIZATION IN 2P25

被引：15

作者：

ANNILO, T

LAAN, M

STAHL, J

METSPALU, A

机构：

[1] TARTU STATE UNIV,ESTONIAN BIOCTR,INST MOLEC & CELL BIOL,TARTU 202400,ESTONIA

[2] HELSINKI UNIV,DEPT CLIN CHEM,HELSINKI,FINLAND

[3] MAX DELBRUCK CENTRUM MOLEK MED,BERLIN,GERMANY

来源：

GENE | 1995年 / 165卷 / 02期

关键词：

HOMO SAPIENS; TRANSCRIPTION INITIATION; FLUORESCENCE IN SITU HYBRIDIZATION; PROTEIN HOMOLOGY;

D O I：

10.1016/0378-1119(95)00576-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have identified a gene encoding the human ribosomal protein (r-protein) S7. The S7 gene contains seven exons and six introns spanning about 6 kb, Organization of the gene is similar to that of Xenopus laevis S8, the only homologous intron-containing gene isolated so far, An mRNA transcribed from this gene has an open reading frame (ORF) of 582 nucleotides (nt), which encodes a protein of 194 amino acids (22.1 kDa). The transcription start point (tsp) was mapped by a primer extension assay to a C residue within a pyrimidine-rich tract. Human S7 (hS7) is identical to rat S7 (rS7) and exhibits significant similarity with the X. laevis, insect and plant homologs. We have used fluorescence in situ hybridization (FISH) to localize S7 to chromosome 2p25.

引用

页码：297 / 302

页数：6

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