DETERMINATION OF L-ALANINE IN A FLOW-INJECTION SYSTEM WITH AN IMMOBILIZED ENZYME REACTOR

被引：5

作者：

KIBA, N

TAGAMI, H

FURUSAWA, M

机构：

[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 239卷 / 02期

关键词：

Alanine; Beverages; Enzyme reactor; Serum;

D O I：

10.1016/S0003-2670(00)83867-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection system for the determination of l-alanine is described. Alanine dehydrogenase is immobilized on poly(vinyl alcohol) beads and used in a packed-bed enzyme reactor. The system responds linearly to injected samples (50 μl) in the concentration range 0.5-500 μM. The maximum throughput was 40 samples per hour. The immobilized enzyme reactor was stable for at least 6 weeks. Its usefulness for assay of l-alanine in serum and beverages is described. © 1990.

引用

页码：307 / 310

页数：4

共 7 条

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