INVITRO SIMULATION OF GASTRIC DIGESTION OF MILK-PROTEINS - COMPARISON BETWEEN INVITRO AND INVIVO DATA

Our previously proposed in vitro model simulating gastric digestion of proteins was automated. Milk digestion products obtained with this automated ''artificial stomach'' were then compared to results obtained in vivo with preruminant calves. By manipulating four essential parameters, emptying rate of fresh matter, pH decrease, nature of enzyme, and enzyme/substrate ratio, the kinetics of gastric emptying of digestive products observed for 6 h in vivo was quantitatively and qualitatively reproduced in vitro in half the time. Therefore, our model could have a great number of applications in the field of human and animal nutrition, particularly in testing the effect of different processing and preparation procedures on the digestion of food.