CHARACTERIZATION OF GENE ORGANIZATION AND PROMOTER REGION OF THE RAT DOPAMINE D1 RECEPTOR GENE

被引:71
作者
ZHOU, QY
LI, C
CIVELLI, O
机构
[1] OREGON HLTH SCI UNIV, VOLLUM INST, L474, 3181 SW SAM JACKSON PK RD, PORTLAND, OR 97201 USA
[2] OREGON HLTH SCI UNIV, DEPT BIOCHEM, PORTLAND, OR 97201 USA
[3] OREGON HLTH SCI UNIV, DEPT CELL BIOL & ANAT, PORTLAND, OR 97201 USA
关键词
DOPAMINE RECEPTOR GENE; 5'-FLANKING SEQUENCE; NEUROBLASTOMA NS20Y CELL; CYCLIC AMP INDUCTION; AUTOREGULATION;
D O I
10.1111/j.1471-4159.1992.tb11023.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genomic and cDNA clones encoding the rat D1 receptor were isolated and sequenced. Comparison of the D1 receptor cDNA and genomic sequences revealed that the rat D1 receptor gene is organized into two exons separated by a small intron in the 5' untranslated region of its mRNA. The transcription start site is located 864 bp upstream from the translational initiation site. The 5'-flanking sequences of the DI receptor gene do not contain TATA and CAAT canonical sequences, but have a high G + C content, potential cyclic AMP and glucocorticoid response element sequences, and binding sites for transcription factors such as Sp1, Ap1, and Ap2. Transfection studies using the D1 5'-flanking sequence and CAT gene fusion constructs have demonstrated that (1) the D1 promoter is active in D1-expressing neuroblastoma NS20Y cells, but inactive in D1-deficient glioma C6 and kidney 293 cells, (2) the information contained within 735 bp of 5'-flanking sequence of the D1 gene appears to be sufficient to confer its cell-specific expression, and (3) the D1 gene promoter responds to cyclic AMP induction, suggesting the existence of an autoregulation mechanism by which the stimulation of D1 receptor exerts a positive feedback on its own gene expression.
引用
收藏
页码:1875 / 1883
页数:9
相关论文
共 52 条
[1]   SCH 23390 BLOCKS DRUG-CONDITIONED PLACE-PREFERENCE AND PLACE-AVERSION - ANHEDONIA (LACK OF REWARD) OR APATHY (LACK OF MOTIVATION) AFTER DOPAMINE-RECEPTOR BLOCKADE [J].
ACQUAS, E ;
CARBONI, E ;
LEONE, P ;
DICHIARA, G .
PSYCHOPHARMACOLOGY, 1989, 99 (02) :151-155
[2]  
BARTON AC, 1990, MOL PHARMACOL, V38, P531
[3]   GENOMIC FOOTPRINTING REVEALS CELL TYPE SPECIFIC DNA-BINDING OF UBIQUITOUS FACTORS [J].
BECKER, PB ;
RUPPERT, S ;
SCHUTZ, G .
CELL, 1987, 51 (03) :435-443
[4]  
BENKIRANE S, 1987, N-S ARCH PHARMACOL, V335, P502
[5]   INHIBITION BY DOPAMINE OF (NA++K+)ATPASE ACTIVITY IN NEOSTRIATAL NEURONS THROUGH D1 AND D2 DOPAMINE RECEPTOR SYNERGISM [J].
BERTORELLO, AM ;
HOPFIELD, JF ;
APERIA, A ;
GREENGARD, P .
NATURE, 1990, 347 (6291) :386-388
[6]   IDENTIFICATION OF A FAMILY OF MUSCARINIC ACETYLCHOLINE-RECEPTOR GENES [J].
BONNER, TI ;
BUCKLEY, NJ ;
YOUNG, AC ;
BRANN, MR .
SCIENCE, 1987, 237 (4814) :527-532
[7]   PURIFICATION AND BIOCHEMICAL-CHARACTERIZATION OF THE PROMOTER-SPECIFIC TRANSCRIPTION FACTOR, SPL [J].
BRIGGS, MR ;
KADONAGA, JT ;
BELL, SP ;
TJIAN, R .
SCIENCE, 1986, 234 (4772) :47-52
[8]   HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].
CHEN, C ;
OKAYAMA, H .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752
[9]   MOLECULAR-BIOLOGY OF THE DOPAMINE-RECEPTORS [J].
CIVELLI, O ;
BUNZOW, JR ;
GRANDY, DK ;
ZHOU, QY ;
VANTOL, HHM .
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1991, 207 (04) :277-286
[10]   REVIEW - D1 DOPAMINE RECEPTOR - THE SEARCH FOR A FUNCTION - A CRITICAL-EVALUATION OF THE D1/D2 DOPAMINE RECEPTOR CLASSIFICATION AND ITS FUNCTIONAL IMPLICATIONS [J].
CLARK, D ;
WHITE, FJ .
SYNAPSE, 1987, 1 (04) :347-388