A METHOD FOR PREPARING ANALYTICALLY PURE SODIUM DITHIONITE - DITHIONITE QUALITY AND OBSERVED NITROGENASE-SPECIFIC ACTIVITIES

被引：64

作者：

MCKENNA, CE

GUTHEIL, WG

WEI, S

机构：

[1] Department of Chemistry, University of Southern California, Los Angeles, CA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1075卷 / 01期

关键词：

SODIUM DITHIONITE; RECRYSTALLIZATION; NITROGENASE; REDOX ENZYME; (A-VINELANDII);

D O I：

10.1016/0304-4165(91)90082-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sodium dithionite (Na2S2O4) is widely used as a reductant in biochemical studies, but has not been available in its pure form. A convenient, detailed procedure is given for the recrystallization of commercial dithionite from 0.1 M NaOH-methanol under anaerobic conditions. Twice-recrystallized dithionite had a purity of 99 +/- 1% by UV spectroscopy (A315) and elemental analysis. The influence of dithionite quality on the apparent reduction activities of the nitrogenase components (Av1 and Av2) from Azotobacter vinelandii was investigated.

引用

页码：109 / 117

页数：9

共 43 条

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