This work investigates the effect of hypothalamic paraventricular nucleus(PVN) on acupuncture analgesia in the rat. Electrical stimulation of PVN or injection of L-glutamate sodium into PVN could enhance the analgesic effect induced by acupuncture Zusanli(St. 36) while the electrical cauterization of PVN is decreased;Removal of the pituitary could not influence the effect of enhancing acupuncture analgesia induced by the injection of L-glutamate sodium into PVN. These results suggest that PVN might play an important role in acupuncture analgesia through central nervous system.