REGULATION OF L-ALANINE-INITIATED GERMINATION OF BACILLUS-SUBTILIS SPORES BY ALANINE RACEMASE

被引:16
作者
YASUDA, Y
KANDA, K
NISHIOKA, S
TANIMOTO, Y
KATO, C
SAITO, A
FUKUCHI, S
NAKANISHI, Y
TOCHIKUBO, K
机构
[1] Department of Microbiology, Nagoya City University Medical School, Mizuho-Ku, 467, Nagoya
关键词
AMINO ACIDS; L-ALA; BACILLUS-SUBTILIS; SPORE; GERMINATION; ALANINE RACEMASE;
D O I
10.1007/BF00805804
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Germination of Bacillus subtilis spores was initiated by L-Ala and competitively inhibited by D-Ala, suggesting the presence of an alanine receptor. The spores showed alanine racemase activity in the spore coat. To investigate the role of alanine racemase (L --> D) on germination, net racemase activity was determined using diphenylamine as a germination inhibitor and germination was measured using D-penicillamine as a racemase inhibitor. Apparent affinity of L-Ala to the germinant receptor was more than 1000 times higher than that to the racemase. Germination increased in the presence of D-penicillamine, when the concentration of L-Ala was low and that of spores was high. Racemase activity was optimal at 65-degrees-C at pH 9.0 and germination at 43-degrees-C at pH 7.2. Under unfavorable growth conditions such as high population of spores in limited nutrients, high temperature and high pH, spore alanine racemase converted the germinant actively to the inhibitor and this conversion may regulate germination for survival of the population.
引用
收藏
页码:89 / 99
页数:11
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