PHAGE LIBRARIES FOR GENERATION OF CLINICALLY USEFUL ANTIBODIES

被引：158

作者：

CHESTER, KA

BEGENT, RHJ

ROBSON, L

KEEP, P

PEDLEY, RB

BODEN, JA

BOXER, G

GREEN, A

WINTER, G

COCHET, O

HAWKINS, RE

机构：

[1] ROYAL FREE HOSP,SCH MED,LONDON NW3 2PF,ENGLAND

[2] CTR PROT ENGN,CAMBRIDGE,CAMBS,ENGLAND

[3] ADDENBROOKES HOSP,CTR PROT ENGN,CAMBRIDGE,CAMBS,ENGLAND

[4] ADDENBROOKES HOSP,DEPT CLIN ONCOL,CAMBRIDGE,CAMBS,ENGLAND

来源：

LANCET | 1994年 / 343卷 / 8895期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0140-6736(94)92695-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Insertion of antibody genes into filamentous bacteriophage makes it possible to generate and screen libraries of 10(7) or more antibodies. Each phage expresses an antibody on its surface and contains the corresponding antibody gene. Genes that encode antibodies with desired characteristics are readily selected and their antibodies expressed as soluble proteins in Escherichia coli. We used this system to produce an antibody to carcinoembryonic antigen with higher affinity and better tumour specificity than antibodies currently in use.

引用

页码：455 / 456

页数：2

共 6 条

[1]

BEGENT RHJ, 1990, SCI CANCER TREATMENT, V2, P161

[2] MAKING ANTIBODY FRAGMENTS USING PHAGE DISPLAY LIBRARIES [J].