REGULATORY PEPTIDES IN GASTRIC ENDOCRINE-CELLS OF THE RAINBOW-TROUT ONCORHYNCHUS-MYKISS - GENERAL DISTRIBUTION AND COLOCALIZATIONS

被引：33

作者：

BARRENECHEA, MA ^{[1
]}

LOPEZ, J ^{[1
]}

MARTINEZ, A ^{[1
]}

机构：

[1] UNIV NAVARRA, DEPT CYTOL & HISTOL, E-31080 PAMPLONA, SPAIN

来源：

TISSUE & CELL | 1994年 / 26卷 / 03期

关键词：

ENDOCRINE CELLS; STOMACH; REGULATORY PEPTIDES; CHROMOGRANIN; SOMATOSTATIN; 25; TELEOSTS; ONCORHYNCHUS MYKISS;

D O I：

10.1016/0040-8166(94)90017-5

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The endocrine cells of the rainbow trout (Oncorhynchus mykiss) stomach have been investigated using the immunocytochemical techniques of peroxidase-anti-peroxidase and avidin-biotin-peroxidase complexes on paraffin sections. 33 antisera were tested and eight immunoreactivities were detected: somatostatin-, glucagon- bombesin-, substance P-, serotonin-, met-enkephalin-, CCK/gastrin-, and chromogranin-like containing cells. All of them were present throughout the gastric mucosa except CCK/gastrin-like containing cells that were restricted to the pyloric epithelium. Somatostatin 25 and chromogranin immunoreactive cells are described for the first time in fish stomach. Serotonin immunoreactive cells were also positive for the Grimelius technique and some of them were immunoreactive to anti substance P or anti CCK/gastrin. Immunoreactivities for gastrin 17, gastrin 34 and CCK appeared in the same cells and the absorption controls showed that a molecule containing the carboxi-terminal pentapeptide of this family was present in trout stomach.

引用

页码：309 / 321

页数：13

共 37 条

[1] A COMPARATIVE IMMUNOCYTOCHEMICAL STUDY OF THE GASTROENTEROPANCREATIC (GEP) ENDOCRINE SYSTEM IN A STOMACHLESS AND A STOMACH-CONTAINING TELEOST [J].

ABAD, ME ;

BINKHORST, FMP ;

ELBAL, MT ;

ROMBOUT, JHWM .

GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1987, 66 (01) :123-136

[2]

ANDERSON C, 1988, CELL TISSUE RES, V254, P553

[3] ENDOCRINE-CELLS AND NERVES IN THE PYLORIC CECA AND THE INTESTINE OF ONCORHYNCHUS-MYKISS (TELEOSTEI) - AN IMMUNOCYTOCHEMICAL STUDY [J].

BEORLEGUI, C ;

MARTINEZ, A ;

SESMA, P .

GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1992, 86 (03) :483-495

[4] SOME PEPTIDE-LIKE COLOCALIZATIONS IN ENDOCRINE-CELLS OF THE PYLORIC CECA AND THE INTESTINE OF ONCORHYNCHUS-MYKISS (TELEOSTEI) [J].

BEORLEGUI, C ;

MARTINEZ, A ;

SESMA, P .

CELL AND TISSUE RESEARCH, 1992, 269 (02) :353-357

[5] NEUROPEPTIDES IN THE FISH GUT - AN IMMUNOHISTOCHEMICAL STUDY OF EVOLUTIONARY PATTERNS [J].

BJENNING, C ;

HOLMGREN, S .

HISTOCHEMISTRY, 1988, 88 (02) :155-163

[6]

BUFFA R, 1988, BASIC APPL HISTOCHEM, V32, P471

[7]

BURKHARDTHOLM P, 1989, CELL TISSUE RES, V255, P245

[8] CHROMOGRANIN-A (CGA) IN THE GASTROENTEROPANCREATIC (GEP) ENDOCRINE SYSTEM .2. CGA IN MAMMALIAN ENTERO-ENDOCRINE CELLS [J].

CETIN, Y ;

MULLERKOPPEL, L ;

AUNIS, D ;

BADER, MF ;

GRUBE, D .

HISTOCHEMISTRY, 1989, 92 (04) :265-275

[9] CO-LOCALIZATION OF SUBSTANCE P-LIKE, BOMBESIN-LIKE AND PEPTIDE HISTIDINE ISOLEUCINE (PHI)-LIKE PEPTIDES IN GUT ENDOCRINE-CELLS OF THE DOGFISH SCYLIORHINUS-STELLARIS [J].

CIMINI, V ;

VANNOORDEN, S ;

POLAK, JM .

ANATOMY AND EMBRYOLOGY, 1989, 179 (06) :605-614

[10] SCYLIORHININ-I AND SCYLIORHININ-II - 2 NOVEL TACHYKININS FROM DOGFISH GUT [J].

CONLON, JM ;

DEACON, CF ;

OTOOLE, L ;

THIM, L .

FEBS LETTERS, 1986, 200 (01) :111-116

← 1 2 3 4 →