A HIGH-RESOLUTION LIQUID CHROMATOGRAPH

A description is given of an apparatus for high resolution liquid chromatography which can be used for the analysis of high molecular weight substances, thermally labile compounds, and reactive products which cannot be analyzed by gas chromatography. A pulseless high pressure pump capable of generating pressures up to 1000 psi is used. The sample is introduced into the stream using a specially designed injector system. After separation on the chromatographic column, the eluted components are detected by determining the ultraviolet absorption of the stream in a 20µl flow-through cell with a 1-cm path length. Chromatograms are shown of the separation of coronene, benzo[a]pyrene, and phenanthrene on two different columns. © 1968, American Chemical Society. All rights reserved.