SOLUBILIZED AND INSOLUBILIZED BONE MORPHOGENETIC PROTEIN

被引：432

作者：

URIST, MR

MIKULSKI, A

LIETZE, A

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 04期

关键词：

D O I：

10.1073/pnas.76.4.1828

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A bone morphogenetic protein (BMP) obtained in solution by digestion of demineralized rabbit cortical bone matrix with bacterial collagenase retains its biologically active conformation in a neutral salt-ethylene glycol mixture. BMP may be insolubilized by coprecipitation with calcium phosphate and resolubilized by chemical extraction with a neutral salt in the same solvent mixture. Upon concanavalin A-Sepharose chromatography, BMP is bound by hydrophobic interaction and carbohydrate recognition and is recovered by elution with either α-methyl mannoside or ethylene glycol solvent mixture. Implants of both eluates and the extracts of the coprecipitate in double-walled diffusion chambers induce transmembrane bone morphogenesis. BMP is not species specific, rabbit BMP induces new bone formation in the rat. The present observations indicate that BMP is a glycoprotein.

引用

页码：1828 / 1832

页数：5

共 33 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN FROM BOVINE CORTICAL BONE [J].