NONRADIOACTIVE LABELING OF OLIGONUCLEOTIDES INVITRO WITH THE HAPTEN DIGOXIGENIN BY TAILING WITH TERMINAL TRANSFERASE

被引：323

作者：

SCHMITZ, GG ^{[1
]}

WALTER, T ^{[1
]}

SEIBL, R ^{[1
]}

KESSLER, C ^{[1
]}

机构：

[1] BOEHRINGER MANNHEIM GMBH, BIOCHEM RES CTR, NONNENWALD 2, W-8122 PENZBERG, GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90212-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the nonradioactive labeling of oligonucleotides with the hapten digoxigenin (DIG) has been developed. The label is introduced by enzymatic tailing of the 3′-end. Two different modified nucleotides were applied. DIG-dUTP allows the synthesis of hapten-modified oligonucleotides with longer tails containing several DIG molecules, whereas the novel compound DIG-ddUTP leads to the addition of only a single DIG hapten. The efficiency of the labeling reactions with respect to variation of the different parameters was analyzed and data on application of labeled oligonucleotide probes to different blot formats are shown. © 1991.

引用

页码：222 / 231

页数：10

共 61 条

[1] HINDERED DIALKYLAMINO NUCLEOSIDE PHOSPHITE REAGENTS IN THE SYNTHESIS OF 2 DNA 51-MERS
ADAMS, SP
KAVKA, KS
WYKES, EJ
HOLDER, SB
GALLUPPI, GR
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (03) : 661 - 663
[2] INSITU HYBRIDIZATION HISTOCHEMISTRY - LOCALIZATION OF VASOPRESSIN MESSENGER-RNA IN RAT-BRAIN USING A BIOTINYLATED OLIGONUCLEOTIDE PROBE
ARAI, H
EMSON, PC
AGRAWAL, S
CHRISTODOULOU, C
GAIT, MJ
[J]. MOLECULAR BRAIN RESEARCH, 1988, 4 (01): : 63 - 69
[3] Ausubel F. M., 1987, MOL REPROD DEV, DOI DOI 10.1002/MRD.1080010210
[4] IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS
BLUM, H
BEIER, H
GROSS, HJ
[J]. ELECTROPHORESIS, 1987, 8 (02) : 93 - 99
[5] A GENERAL METHOD FOR ISOLATION OF RNA COMPLEMENTARY TO DNA
BOLTON, ET
MCCARTHY, BJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (08) : 1390 - &
[6] CANTOR CR, 1980, CONFORMATION BIOL 1, P115
[7] AN RNA POLYMERASE-II TRANSCRIPTION FACTOR BINDS TO AN UPSTREAM ELEMENT IN THE ADENOVIRUS MAJOR LATE PROMOTER
CARTHEW, RW
CHODOSH, LA
SHARP, PA
[J]. CELL, 1985, 43 (02) : 439 - 448
[8] SYNTHESIS AND HYBRIDIZATION OF A SERIES OF BIOTINYLATED OLIGONUCLEOTIDES
COOK, AF
VUOCOLO, E
BRAKEL, CL
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (09) : 4077 - 4095
[9] DAVEY C, 1988, Patent No. 272098
[10] RAPID DETECTION OF DNA-BINDING FACTORS USING PROTEIN-BLOTTING AND DIGOXYGENINE-DUTP MARKED PROBES
DOOLEY, S
RADTKE, J
BLIN, N
UNTEREGGER, G
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (24) : 11839 - 11839

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