A NUCLEAR-MAGNETIC-RESONANCE TECHNIQUE FOR DETERMINING HYBRIDOMA CELL CONCENTRATION IN HOLLOW FIBER BIOREACTORS

被引：42

作者：

MANCUSO, A ^{[1
]}

FERNANDEZ, EJ ^{[1
]}

BLANCH, HW ^{[1
]}

CLARK, DS ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, DEPT CHEM ENGN, BERKELEY, CA 94720 USA

来源：

BIO-TECHNOLOGY | 1990年 / 8卷 / 12期

关键词：

D O I：

10.1038/nbt1290-1282

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a technique for determining cell concentration in a hollow fiber bioreactor based on 23Na nuclear magnetic resonance (NMR) spectroscopy. Cell concentrations determined with this method agreed closely with concentrations calculated from 31P NMR nucleoside triphosphate (NTP) measurements and oxygen consumption rate measurements. Oxygen transfer limitations, which can complicate cell mass determinations based on oxygen consumption rates, were shown to be negligible for the bioreactor used. Specific antibody production rates in hollow fiber culture, calculated from these cell number estimates, were similar to those found in suspension culture for this cell line. © 1990 Nature Publishing Group.

引用

页码：1282 / 1285

页数：4

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