A H-1-NMR METHOD FOR DETERMINING TEMPERATURE IN CELL-CULTURE PERFUSION SYSTEMS

被引：50

作者：

LUTZ, NW ^{[1
]}

KUESEL, AC ^{[1
]}

HULL, WE ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,DEPT CENT SPECT,NEUEMHEIMER FELD 280,W-6900 HEIDELBERG,GERMANY

来源：

MAGNETIC RESONANCE IN MEDICINE | 1993年 / 29卷 / 01期

关键词：

H-1-NMR; TEMPERATURE; CELL CULTURE; PERFUSION;

D O I：

10.1002/mrm.1910290120

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

This report describes a noninvasive H-1-NMR method for measuring absolute temperatures (+/- 0.2-degrees-C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the components of the biological medium, e.g., pyruvate, acetate, or lactate. The effects of flow on temperature can be monitored and appropriate adjustments of the temperature controller can be made.

引用

页码：113 / 118

页数：6

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