BOVINE RESPIRATORY SYNCYTIAL VIRUS-SPECIFIC IMMUNE-RESPONSES IN CATTLE FOLLOWING IMMUNIZATION WITH MODIFIED-LIVE AND INACTIVATED VACCINES - ANALYSIS OF THE SPECIFICITY AND ACTIVITY OF SERUM ANTIBODIES

被引：20

作者：

ELLIS, JA ^{[1
]}

RUSSELL, H ^{[1
]}

CAVENDER, J ^{[1
]}

HAVEN, TR ^{[1
]}

机构：

[1] UNIV WYOMING,DEPT VET SCI,LARAMIE,WY 82070

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1992年 / 34卷 / 1-2期

关键词：

D O I：

10.1016/0165-2427(92)90150-O

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cattle were immunized with vaccines containing modified-live or inactivated bovine respiratory syncytial virus (BRSV) and serum antibody responses were analyzed. Compared with preinculation values, at Day 14 after two biweekly immunizations with modified-live or inactivated vaccines there were significant increases in BRSV-specific titers in the sera of cattle that received both types of vaccines, as determined by a whole cell ELISA. Using a blocking ELISA and radioimmune precipitation it was determined that there was recognition of the fusion (F) protein by antibodies from cattle that received both types of BRSV antigens: however, virus neutralization assays revealed that only cattle that received modified live virus, either in monovalent or polyvalent vaccines, developed neutralizing antibodies to BRSV after two immunizations. These results indicate that inactivation of BRSV can lead to a dissociation between serological recognition of the F protein and virus neutralization in vaccinated cattle.

引用

页码：35 / 45

页数：11

共 25 条

[1] THE ROLE OF PASSIVE-IMMUNITY IN BOVINE RESPIRATORY SYNCYTIAL VIRUS-INFECTED CALVES [J].