INTRACELLULAR PH AND BUFFERING POWER MEASURED IN ISOLATED SINGLE CELLS FROM PREGNANT RAT UTERUS

被引：15

作者：

EIESLAND, J

BARO, I

RAIMBACH, S

EISNER, DA

WRAY, S

机构：

[1] UNIV LIVERPOOL, DEPT PHYSIOL, POB 147, LIVERPOOL L69 3BX, ENGLAND

[2] UNIV LIVERPOOL, DEPT VET PRECLIN SCI, LIVERPOOL L69 3BX, ENGLAND

来源：

EXPERIMENTAL PHYSIOLOGY | 1991年 / 76卷 / 05期

关键词：

D O I：

10.1113/expphysiol.1991.sp003548

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Intracellular pH (pHi) affects smooth muscle function yet there have been few direct pHi measurements. Using the fluorescent indicator, 2',7'-bis-2-(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester, we report here the first measurements of pHi and buffering power (beta) in single, isolated, uterine smooth muscle cells. Mean pHi in cells from pregnant rat uteri was 7.09 +/- 0.08 (+/- S.E.M., n = 16 cells; 37-degrees-C), and beta was found to be 12.8 +/- 2.8 mmol/l per pH unit (n = 15). The cells were able to regulate their pHi in the presence of butyrate when perfused in nominally CO2 - free solution.

引用

页码：815 / 818

页数：4

共 7 条

[1]

AICKIN CC, 1988, CIBA F SYMP, V139, P3

[2] ISOLATION AND CONTRACTILE RESPONSES OF SINGLE PREGNANT RAT MYOMETRIAL CELLS IN SHORT-TERM PRIMARY CULTURE AND THE EFFECTS OF PHARMACOLOGICAL AND ELECTRICAL STIMULI [J].