GIBBERELLIN-A(82) AND GIBBERELLIN-A(83) IN SEED OF LUPINUS-ALBUS

Extracts from seeds of Lupinus albus at 14, 22, 35 and 52 days after anthesis were separated into free gibberellins, ester conjugates and ether conjugates. Capillary GC-MS of the methylated and trimethylsilylated free gibberellin fractions showed the presence of the known gibberellins A1, A3, A4, A17, A18, A23 and A43. In addition six new gibberellin-like compounds were detected that corresponded to the addition of the elements of water to gibberellins A3, A4, A7, A14, A18 and A34. Two of these components were identified by chemical syntheses as ent-3-alpha,10-beta,17-trihydroxy-20-nor-16-alpha-Hgibberellane-7,19-dioic acid 19,10-lactone and ent-3-alpha,17-dihydroxy-16-alpha-Hgibberellane-7,19-dioic acid, which are accorded the gibberellin numbers A82 and A83, respectively. ent-3-alpha,10-beta,16-beta,17-Tetrahydroxy-20-nor-16-beta-Hgibberellane-7,19-dioc acid 19,10-lactone was also identified by synthesis of the methyl ester. Similar analyses of the hydrolysed ether conjugate fractions showed the presence of the known gibberellins A1, A3, A13, A18 and A43, the new gibberellin A82 and the 'hydrated' gibberellins A18 and A34; the 15-ene isomers of gibberellins A13 and A43 and the 16-xi-17-epoxide of gibberellin A18 were also identified as probable artefacts. In the hydrolysed ester conjugate fractions the new gibberellin A82 and the 'hydrated' gibberellin A34 were detected. Gibberellin A18 was by far the most abundant GA but quantitation of the GAs was not carried out.