PEPTIDE IMMUNOREACTIVE NEURONS IN THE AMYGDALA AND THE BED NUCLEUS OF THE STRIA TERMINALIS PROJECT TO THE MIDBRAIN CENTRAL GRAY IN THE RAT

被引:214
作者
GRAY, TS
MAGNUSON, DJ
机构
[1] Department of Cell Biology, Neurobiology and Anatomy, Loyola Stritch School of Medicine, Maywood, IL 60153
关键词
CORTICOTROPIN RELEASING FACTOR; NEUROTENSIN; SOMATOSTATIN; SUBSTANCE-P; IMMUNOCYTOCHEMISTRY; RETROGRADE TRACING;
D O I
10.1016/0196-9781(92)90074-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The central nucleus of the amygdala, bed nucleus of the stria terminalis, and central gray are important components of the neural circuitry responsible for autonomic and behavioral responses to threatening or stressful stimuli. Neurons of the amygdala and bed nucleus of the stria terminalis that project to the midbrain central gray were tested for the presence of peptide immunoreactivity. To accomplish this aim, a combined immunohistochemical and retrograde tracing technique was used. Maximal retrograde labeling was observed in the amygdala and bed nucleus of the stria terminalis after injections of retrograde tracer into the caudal ventrolateral midbrain central gray. The majority of the retrogradely labeled neurons in the amygdala were located in the medial central nucleus, although many neurons were also observed in the lateral subdivision of the central nucleus. Most of the retrogradely labeled neurons in the BST were located in the ventral and posterior lateral subdivisions, although cells were also observed in most other subdivisions. Retrogradely labeled neurotensin, corticotropin releasing factor (CRF), and somatostatin neurons were mainly observed in the lateral central nucleus and the dorsal lateral BST. Retrogradely labeled substance P-immunoreactive cells were found in the medial central nucleus and the posterior and ventral lateral BST. Enkephalin-immunoreactive retrogradely labeled cells were not observed in the amygdala or bed nucleus of the stria terminalis. A few cells in the hypothalamus (paraventricular and lateral hypothalamic nuclei) that project to the central gray also contained CRF and neurotensin immunoreactivity. The results suggest the amygdala and the bed nucleus of the stria terminalis are a major forebrain source of CRF, neurotensin, somatostatin, and substance P terminals in the midbrain central gray.
引用
收藏
页码:451 / 460
页数:10
相关论文
共 33 条
[1]   ACTIVE MUSCLE VASODILATATION PRODUCED BY STIMULATION OF THE BRAIN STEM - ITS SIGNIFICANCE IN THE DEFENCE REACTION [J].
ABRAHAMS, VC ;
HILTON, SM ;
ZBROZYNA, A .
JOURNAL OF PHYSIOLOGY-LONDON, 1960, 154 (03) :491-513
[2]   ELICITATION OF INTRASPECIFIC DEFENSE REACTIONS IN THE RAT FROM MIDBRAIN PERIAQUEDUCTAL GREY BY MICROINJECTION OF KAINIC ACID, WITHOUT NEUROTOXIC EFFECTS [J].
BANDLER, R ;
DEPAULIS, A .
NEUROSCIENCE LETTERS, 1988, 88 (03) :291-296
[3]   A MECHANISM FOR THE ANALGESIC EFFECT OF NEUROTENSIN AS REVEALED BY BEHAVIORAL AND ELECTROPHYSIOLOGICAL TECHNIQUES [J].
BEHBEHANI, MM ;
PERT, A .
BRAIN RESEARCH, 1984, 324 (01) :35-42
[4]   PEPTIDE INJECTIONS INTO THE AMYGDALA OF CONSCIOUS RATS - EFFECTS ON BLOOD-PRESSURE, HEART-RATE AND PLASMA-CATECHOLAMINES [J].
BROWN, MR ;
GRAY, TS .
REGULATORY PEPTIDES, 1988, 21 (1-2) :95-106
[5]  
BROWN MR, 1991, NEUROBIOLOGY NEUROEN, P193
[6]   SLEEP STATES ATTENUATE THE PRESSOR-RESPONSE TO CENTRAL AMYGDALA STIMULATION [J].
FRYSINGER, RC ;
MARKS, JD ;
TRELEASE, RB ;
SCHECHTMAN, VL ;
HARPER, RM .
EXPERIMENTAL NEUROLOGY, 1984, 83 (03) :604-617
[7]   HEMODYNAMIC-RESPONSES TO AMYGDALOID STIMULATION IN SPONTANEOUSLY HYPERTENSIVE RATS [J].
GALENO, TM ;
BRODY, MJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1983, 245 (02) :R281-R286
[8]   NEUROPEPTIDE NEURONAL EFFERENTS FROM THE BED NUCLEUS OF THE STRIA TERMINALIS AND CENTRAL AMYGDALOID NUCLEUS TO THE DORSAL VAGAL COMPLEX IN THE RAT [J].
GRAY, TS ;
MAGNUSON, DJ .
JOURNAL OF COMPARATIVE NEUROLOGY, 1987, 262 (03) :365-374
[9]  
GRAY TS, 1987, NEUROSCI LETT, V83, P163
[10]  
GRAY TS, 1991, STRESS NEUROBIOLOGY, P73