RECEPTORS FOR TUMOR-PROMOTING PHORBOL ESTERS IN RAT VENTRAL PROSTATE

被引：4

作者：

CARMENA, MJ ^{[1
]}

GARCIAPARAMIO, MP ^{[1
]}

PRIETO, JC ^{[1
]}

机构：

[1] UNIV ALCALA DE HENARES, DEPT BIOQUIM & BIOL MOLEC, E-28871 ALCALA DE HENARES, SPAIN

来源：

CANCER LETTERS | 1993年 / 68卷 / 2-3期

关键词：

TUMOR-PROMOTING PHORBOL ESTER BINDING; PROTEIN KINASE-C; SIGNAL TRANSDUCTION;

D O I：

10.1016/0304-3835(93)90140-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The presence of tumor-promoting phorbol ester receptors in rat prostate was investigated by studying the binding of phorbol diester 12,13-dibutyrate (PDBu) in both soluble and particulate subcellular fractions. Binding of [H-3]PDBu to the soluble fraction was optimal after the addition of phosphatidylserine (0.1 mg/ml) and Ca2+ (1 mM). Both subcellular fractions exhibited a single class of PDBu receptor (K(d) between 97 and 128 nM) as shown by saturation binding experiments. Phorbol esters with tumor-promoting activity showed a higher affinity for these receptors than did endogenous ligands such as diacyglycerols whereas phorbol esters without tumor-promoting activity were ineffective even at concentrations as high as 1 muM. These properties are highly representative of protein kinase C activity.

引用

页码：143 / 147

页数：5

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