PHOSPHOLIPIDS OF BARLEY-GRAIN

Fractionation of the phospholipids of two barley (Hordeum vulgare L.) varieties, —Kearney– (winter type) and —Prilar–(spring type), by thin-layer chromatography produced ten lipid classes. Phosphorus analysis of each fraction showed that phosphatidylcholine, lysophosphatidylcholine, and phosphatidylethanolamine were present in highest amounts. Lesser amounts of phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, and small amounts of phosphatidic acid and two unknown phospholipids were present.Fatty acids present in the fractions ranged from lauric (12:0) to arachidic (20:0). Linoleic acid (18:2) was the principal fatty acid in seven fractions and palmitic (16:0) was predominant in the other three fractions. This study provides a basis for monitoring of changes in phospholipids during cold acclimation of barley seedlings. © 1979, American Chemical Society. All rights reserved.