EXPRESSION OF A DOWNSTREAM GENE FROM A BICISTRONIC TRANSCRIPTION UNIT IN TRANSGENIC TOBACCO PLANTS

被引：5

作者：

IIDA, S ^{[1
]}

SCHEID, OM ^{[1
]}

SAUL, MW ^{[1
]}

SEIPEL, K ^{[1
]}

MIYAZAKI, C ^{[1
]}

POTRYKUS, I ^{[1
]}

机构：

[1] FED INST TECHNOL,INST PLANT SCI,ZURICH,SWITZERLAND

来源：

GENE | 1992年 / 119卷 / 02期

基金：

日本学术振兴会;

关键词：

GENE EXPRESSION; HYGROMYCIN; KANAMYCIN; RESISTANCE-ENCODING GENE; PLANT TRANSFORMATION; RECOMBINANT DNA; SELECTIVE MARKER; TRANSLATION;

D O I：

10.1016/0378-1119(92)90272-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a set of plasmids carrying an artificial compact stop-start codon sequence, TGATGTAACATGA, between an upstream open reading frame, terminating at one of the stop codons, and a downstream kanamycin-resistance (Km(R))-encoding gene (nptII) initiating at the second ATG. These plasmids were introduced into tobacco protoplasts by direct gene transfer. The efficiency of expression of the downstream nptII gene was measured by scoring the number of Km(R) transformants. With a closer distance between the functional stop and start codons, a tendency to less efficient expression of nptII was found. The integration and expression of both genes as a bicistronic transcription unit were verified by Southern- and Northern-blot analyses. A possible application of the compact stop-start codon sequence for insertional mutagenesis is discussed.

引用

页码：199 / 205

页数：7

共 18 条

[1] EXPRESSION OF DICISTRONIC TRANSCRIPTIONAL UNITS IN TRANSGENIC TOBACCO [J].