CLONAL ANALYSIS OF ERYTHROPOIETIN STIMULATED J2E CELLS REVEALS ASYNCHRONY DURING TERMINAL DIFFERENTIATION

被引：14

作者：

BUSFIELD, SJ ^{[1
]}

FARR, TJ ^{[1
]}

SINGH, T ^{[1
]}

SAINSBURY, AJ ^{[1
]}

KLINKEN, SP ^{[1
]}

机构：

[1] UNIV WESTERN AUSTRALIA,DEPT BIOCHEM,NEDLANDS,WA 6009,AUSTRALIA

来源：

GROWTH FACTORS | 1993年 / 9卷 / 04期

基金：

澳大利亚研究理事会;

关键词：

ERYTHROPOIETIN; ERYTHROPOIESIS; DIFFERENTIATION; PROLIFERATION;

D O I：

10.3109/08977199308991591

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

J2E erythroid cells proliferate and differentiate in response to erythropoietin (epo), the red blood cell specific hormone. Using methylcellulose colony assays and suspension cultures we have demonstrated that nearly all the cells stimulated by epo synthesized haemoglobin. To achieve maximum production of haemoglobin J2E cells had to be treated with epo for only 6 h; hormone added subsequently did not enhance haemoglobin synthesis. Although virtually all viable J2E cells produced haemoglobin, the cells matured morphologically at different rates. Thus, upon exposure to epo J2E cells become committed to erythroid terminal differentiation but proceed in an asynchronous manner.

引用

页码：307 / 315

页数：9

共 26 条

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