CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF HUMAN PLASMA APOLIPOPROTEIN C-I

Human apolipoprotein C-I, the smallest of the plasma apolipoproteins, is a component of several classes of plasma lipoproteins. Two crystalline forms of this protein, both suitable for high resolution X-ray diffraction analysis, have been obtained from mixtures of 2-methyl-2,4-pentanediol, sodium acetate, and octyl-β-D-1-thioglucopyranoside at room temperature. The first form belongs to space group P21, with β = 106° and cell dimensions of a = 38.1 Å, b = 50.8 Å and c = 34.9 Å. The asymmetric unit appears to contain two molecules of the protein. The second form belongs to space group P212121, with cell dimensions of a = 34.5 Å, b = 53.1 Å, c = 71.1 Å and it also appears to contain two molecules of the protein in the asymmetric unit. © 1994 Academic Press Limited.