STRUCTURAL DIFFERENCES BETWEEN COMPLEX-TYPE ASN-LINKED GLYCAN CHAINS OF GLYCOPROTEINS IN RAT HEPATOCYTES AND ZAJDELA HEPATOMA-CELLS

被引:8
作者
GOULUTCHASSAING, C [1 ]
BOURRILLON, R [1 ]
机构
[1] UFR BIOMED ST PERES, BIOCHIM CELLULAIRE LAB, F-75006 PARIS, FRANCE
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1244卷 / 01期
关键词
GLYCOPROTEIN; N-GLYCAN CHAIN; MALIGNANT TRANSFORMATION; STRUCTURAL ALTERATION; (RAT NORMAL CELL); (RAT TUMOR CELL);
D O I
10.1016/0304-4165(94)00191-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using serial lectin-affinity chromatography, glycosidase digestion, and NMR and methylation analysis, the structures of complex N-linked glycan chains (M(r) range 2000-3500) of rat hepatocytes and poorly differentiated chemically transformed Zajdela ascites hepatoma cells were determined and compared. The results revealed considerable differences between the two cell types: (i) hepatoma cells only expressed tri- and/or tetra-antennary complex N-linked glycan chains, whereas hepatocytes displayed large amounts of bi-antennary N-linked structures and smaller amounts of tri-/tetra-antennary structures; (ii) 20% of the glycan chains in hepatoma cells contained a bisecting GlcNAc residue which was beta(1,4)-linked to the beta-mannosyl residue of the core and was not detected in the hepatocytes; (iii) hepatoma cells expressed a high proportion of the fucosylated or not GlcNAc beta(1,6) Man alpha 1 --> branch, whereas hepatocytes only contained a little of this branch; (iv) hepatoma cells, but not hepatocytes, exhibited a repeating (Gal beta(1,4) GlcNAc beta(1,3)) sequence characteristic of poly-N-acetyllactosaminoglycans. These glycans were capped by both alpha-galactosyl and sialyl residues; (v) The alpha(2,3)/alpha(2,6)-linkage ratio of sialic acid was significantly higher in hepatoma cells (4/1 vs. 2/1 in hepatocytes); (vi) Only hepatocytes expressed an unusual structure in which a sialyl residue was alpha(2,6)-linked to a GlcNAc residue located within a NeuAc alpha(2,3) Gal beta(1,3) GlcNAc branch which was beta(1,4)-linked to Man alpha 1,3 -->. The differences between these complex N-linked glycan chains in hepatocytes and hepatoma cells seem to be both quantitative and qualitative, since some glycan structures were only present in one cell type.
引用
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页码:30 / 40
页数:11
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