THE COMPLETE AMINO-ACID-SEQUENCE OF CHITINASE-B FROM THE LEAVES OF POKEWEED (PHYTOLACCA-AMERICANA)

被引：11

作者：

TANIGAWA, M ^{[1
]}

YAMAGAMI, T ^{[1
]}

FUNATSU, G ^{[1
]}

机构：

[1] KYUSHU UNIV, FAC AGR, PROT CHEM & ENGN LAB, FUKUOKA 812, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 05期

关键词：

D O I：

10.1271/bbb.59.841

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 tryptic peptides derived from the reduced and S-carboxymethylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29,473Da, Six cysteine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188, From 58-68% sequence homology of PLC-B with five class III chitinases, it was concluded that PLC-B is a basic class III chitinase.

引用

页码：841 / 847

页数：7

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