DETECTION AND ANALYSIS OF UNRESOLVED MULTIPLET CHROMATOGRAPHIC PEAKS

The recognition and description of multiple components in a chromatographic peak requires specialized quantitative techniques when these components represent conformational or positional isomers of the same molecular species. The diagnostic characteristics of the dispersion and displacement differences between experimental and reference peaks are used to demonstrate the detection and determination of up to four components in a single peak. Procedures for determining the intra-component separation factors are illustrated, together with an evaluation of the range and accuracy of these techniques. A new method of detecting the presence of a doublet peak in the absence of a suitable reference peak with a single component is presented. © 1968, American Chemical Society. All rights reserved.