SYNOVIOCYTES SYNTHESIZE, BIND, AND RESPOND TO BASIC FIBROBLAST GROWTH-FACTOR

被引:53
作者
MELNYK, VO
SHIPLEY, GD
STERNFELD, MD
SHERMAN, L
ROSENBAUM, JT
机构
[1] OREGON HLTH SCI UNIV,DEPT MED,L329A,3181 SW SAM JACKSON PK RD,PORTLAND,OR 97201
[2] OREGON HLTH SCI UNIV,DEPT OPHTHALMOL,PORTLAND,OR 97201
[3] OREGON HLTH SCI UNIV,DEPT CELL BIOL & ANAT,PORTLAND,OR 97201
来源
ARTHRITIS AND RHEUMATISM | 1990年 / 33卷 / 04期
关键词
D O I
10.1002/art.1780330405
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Rheumatoid arthritis (RA) is a systemic disease characterized by the destructive proliferation of synovial tissue. It has been suggested that this proliferative lesion resembles a malignancy. Although polypeptide growth factors have been implicated in malignant cell growth, their role in the pathogenesis of proliferative but non‐neoplastic diseases such as RA has not been extensively studied. We tested the hypothesis that the synoviocyte itself may be a source of growth factor activity. We demonstrated that culture supernatants from synoviocytes obtained from patients with RA, osteoarthritis, and traumatic joint disease contain mitogenic activity. This activity has biologic properties identical to those of basic fibroblast growth factor (bFGF). Specifically, the mitogenic activity is synergistic with insulin and binds to heparin‐agarose, but elutes with 2.0M NaCl. In addition, synoviocyte extracts contain a peptide with a molecular weight of ∼ 16,000, which reacts with antibody specific for bFGF. Cultured synoviocytes express the bFGF gene, express receptors for bFGF, and proliferate in response to bFGF. We conclude that bFGF derived from the synoviocytes themselves may play a role in stimulating their proliferation in an autocrine manner in disease states such as RA. Copyright © 1990 American College of Rheumatology
引用
收藏
页码:493 / 500
页数:8
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