TOBACCO NUCLEAR GENE FOR THE 31-KD CHLOROPLAST RIBONUCLEOPROTEIN - GENOMIC ORGANIZATION, SEQUENCE-ANALYSIS AND EXPRESSION

被引：32

作者：

LI, YQ ^{[1
]}

YE, LZ ^{[1
]}

SUGITA, M ^{[1
]}

SUGIURA, M ^{[1
]}

机构：

[1] NAGOYA UNIV, CTR GENE RES, CHIKUSA KU, NAGOYA 46401, JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 11期

关键词：

D O I：

10.1093/nar/19.11.2987

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously identified three chloroplast ribonucleoproteins and characterized their cDNAs. Here we present the genomic organization, sequence and expression of one of their genes. The 31 kd ribonucleoprotein (cp31) from tobacco (Nicotiana sylvestris) chloroplasts is coded for by a single-copy nuclear gene. This gene was isolated and its sequence was determined. The gene contains four exons and three introns. The position of its first intron is conserved among the genes for the maize abscisic acid-induced glycine-rich protein, the human hnRNP A1 protein and cp31. The transcription start site was determined to be 168 bp upstream from the translational initiation codon in both leaf and root tissues. No alternatively spliced transcripts was detected, suggesting that a diversity of chloroplast ribonucleoproteins in generated probably by gene amplification rather than alternative splicing.

引用

页码：2987 / 2991

页数：5

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