ACIDIC GLYCOSPHINGOLIPIDS OF HUMAN AMNION

被引：7

作者：

ASANO, K

SUZUKI, M

HANDA, S

OSHIMA, M

机构：

[1] NATL MED CTR,CLIN RES INST,DIV CLIN BIOCHEM,1-21-1 TOYAMA,SHINJUKU KU,TOKYO 162,JAPAN

[2] TOKYO MED & DENT UNIV,FAC MED,DEPT BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN

[3] TOKYO METROPOLITAN INST MED SCI,DEPT BIOMEMBRANE,BUNKYO KU,TOKYO 113,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1991年 / 109卷 / 01期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123329

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Six major acidic glycosphingolipids were isolated from human amnion using DEAE Sephadex A-25 and silica beads column chromatography. The structures of these glycosphingolipids were determined by methylation analysis, TLC immunostaining and/or negative ion FAB-MS, and were concluded to be II3-alpha-NeuAcLacCer(GM3), IV3-alpha-NeuAcnLc4-Cer (sialyl[alpha-2-3]paragloboside), IV6-alpha-NeuAcnLc4Cer (sialyl[alpha-2-6]paragloboside), IV3-alpha-NeuAcIII4-alpha-FucLc4Cer (sialyl Le(a)), VI3-alpha-NeuAcnLc6Cer (i-ganglioside) and II3-alpha(NeuAc-alpha-2 -->8NeuAc)LacCer (GD3). In addition, several minor glycosphingolipids were detected with specific monoclonal antibodies, including glycolipids with NeuAc-alpha-2-3Gal-beta-1-4GlcNAc-beta-1-or NeuAc-alpha-2-6Gal-beta-1-4GlcNAc-beta-1- determinant. Our results show that the glycosphingolipids of human amnion are characterized by having mainly type II chain analogues and onco-fetal antigens.

引用

页码：106 / 112

页数：7

共 28 条

[1]

[Anonymous], 1977, EUR J BIOCHEM, V79, P11

[2]

ASANO K, 1990, JPN J EXP MED, V60, P299

[3]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

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