RELEASE OF CARROT PLASMA MEMBRANE-ASSOCIATED PHOSPHATIDYLINOSITOL KINASE BY PHOSPHOLIPASE-A2 AND ACTIVATION BY A 70 KDA PROTEIN

被引：19

作者：

GROSS, W ^{[1
]}

YANG, W ^{[1
]}

BOSS, WF ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV, DEPT BOT, RALEIGH, NC 27695 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1134卷 / 01期

基金：

美国国家科学基金会;

关键词：

PHOSPHATIDYLINOSITOL KINASE; PHOSPHOLIPASE-A2; PHOSPHATIDYLINOSITOL; PHOSPHATIDYLINOSITOL MONOPHOSPHATE;

D O I：

10.1016/0167-4889(92)90029-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasma membranes were isolated from carrot (Daucus carota L.) cells grown in suspension culture and treated with phospholipase A2 from snake or bee venom for 10 min. As a result of this treatment, phosphatidylinositol kinase activity was recovered in the soluble fraction. There was no detectable diacylglycerol kinase or phosphatidylinositol monophosphate kinase activity released from the membranes after the phospholipase A2 treatment. Treating the plasma membranes with phospholipase C or D did not release PI kinase activity. The phospholipase A2-released PI kinase was activated over 2-fold by a heat stable, soluble 70 kDa protein. The partially purified 70 kDa activator increases the V(max) but does not affect the K(m) of the phospholipase A2-released PI kinase.

引用

页码：73 / 80

页数：8

共 45 条

[1]

BELUNIS CJ, 1988, J BIOL CHEM, V263, P18897

[2]

BERGER M, 1986, J BIOL CHEM, V261, P4912

[3] INOSITOL PHOSPHATES AND CELL SIGNALING [J].