INHIBITION OF HEMATOPOIETIC DEVELOPMENT FROM EMBRYONIC STEM-CELLS BY ANTISENSE VAV RNA

被引：52

作者：

WULF, GM ^{[1
]}

ADRA, CN ^{[1
]}

LIM, B ^{[1
]}

机构：

[1] HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,DEPT MED,DIV HEMATOL & ONCOL,BOSTON,MA 02215

来源：

EMBO JOURNAL | 1993年 / 12卷 / 13期

关键词：

ANTISENSE RNA; EMBRYONIC STEM CELLS; HEMATOPOIESIS; VAV PROTOONCOGENE;

D O I：

10.1002/j.1460-2075.1993.tb06200.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The vav proto-oncogene is universally and specifically expressed in hematopoietic cells. vav contains a unique array of motifs allowing the protein to function as a signal transducer and possibly as a transcription factor. Under certain in vitro culture conditions murine embryonic stem cells develop into colonies containing multiple hematopoietic lineages. In embryonic stem cell lines, constitutively expressing high levels of antisense vav transcripts through a stably integrated transgene, differentiation into hematopoietic cells is disrupted. This observation presents the first evidence that vav has a critical role in the development of hematopoietic cells from totipotent cells.

引用

页码：5065 / 5074

页数：10

共 38 条

[1] ADAMS JM, 1992, ONCOGENE, V7, P611
[2] CLONING AND EXPRESSION OF THE MOUSE PGK-1 GENE AND THE NUCLEOTIDE-SEQUENCE OF ITS PROMOTER
ADRA, CN
BOER, PH
MCBURNEY, MW
[J]. GENE, 1987, 60 (01) : 65 - 74
[3] MOUSE GATA-4 - A RETINOIC ACID-INDUCIBLE GATA-BINDING TRANSCRIPTION FACTOR EXPRESSED IN ENDODERMALLY DERIVED TISSUES AND HEART
ARCECI, RJ
KING, AAJ
SIMON, MC
ORKIN, SH
WILSON, DB
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (04) : 2235 - 2246
[4] THE GENE ENCODING THE STEM-CELL ANTIGEN, CD34, IS CONSERVED IN MOUSE AND EXPRESSED IN HEMATOPOIETIC PROGENITOR-CELL LINES, BRAIN, AND EMBRYONIC FIBROBLASTS
BROWN, J
GREAVES, MF
MOLGAARD, HV
[J]. INTERNATIONAL IMMUNOLOGY, 1991, 3 (02) : 175 - 184
[5] PRODUCT OF VAV PROTOONCOGENE DEFINES A NEW CLASS OF TYROSINE PROTEIN-KINASE SUBSTRATES
BUSTELO, XR
LEDBETTER, JA
BARBACID, M
[J]. NATURE, 1992, 356 (6364) : 68 - 71
[6] Chen U, 1992, Dev Immunol, V2, P29, DOI 10.1155/1992/79518
[7] COPPOLA J, 1991, CELL GROWTH DIFFER, V2, P95
[8] ASSOCIATION OF SOS RAS EXCHANGE PROTEIN WITH GRB2 IS IMPLICATED IN TYROSINE KINASE SIGNAL TRANSDUCTION AND TRANSFORMATION
EGAN, SE
GIDDINGS, BW
BROOKS, MW
BUDAY, L
SIZELAND, AM
WEINBERG, RA
[J]. NATURE, 1993, 363 (6424) : 45 - 51
[9] GRB2 MEDIATES THE EGF-DEPENDENT ACTIVATION OF GUANINE-NUCLEOTIDE EXCHANGE ON RAS
GALE, NW
KAPLAN, S
LOWENSTEIN, EJ
SCHLESSINGER, J
BARSAGI, D
[J]. NATURE, 1993, 363 (6424) : 88 - 92
[10] TYROSINE KINASE-STIMULATED GUANINE-NUCLEOTIDE EXCHANGE ACTIVITY OF VAV IN T-CELL ACTIVATION
GULBINS, E
COGGESHALL, KM
BAIER, G
KATZAV, S
BURN, P
ALTMAN, A
[J]. SCIENCE, 1993, 260 (5109) : 822 - 825

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