IN-VIVO PROTON SPECTROSCOPY AND SPECTROSCOPIC IMAGING OF (1-C-13)-GLUCOSE AND ITS METABOLIC PRODUCTS

Metabolism of {1-C-13}-glucose was studied in situ in cat brain using gradient-enhanced proton-detected heteronuclear spectroscopy. Proton detection of {1-C-18}-glucose, {3-C-13}-lactate, 4-{C-13}-glutamine, 4-{C-13}-glutamate and the combined signals 2-{C-13}-glutamate/glutamine and 3-{C-13}-glutamate/glutamine was achieved, despite the fact that some of the associated proton resonances are close to the water signal. Two-dimensional {H-1-C-13}-spectra demonstrate the possibility of in situ spectral assignment with H-1 sensitivity and C-13 resolution. Spectroscopic images of glucose and its metabolic products were also acquired, showing the possibility to study spatial dependence of metabolism.