OPTIMIZATION OF THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF STEROIDS BY THE OVERLAPPING RESOLUTION MAPPING PROCEDURE

被引：5

作者：

CHONG, KY

KHOO, TH

KOO, FS

ONG, CP

LI, SFY

LEE, HK

VENKATESH, B

TAN, CH

机构：

[1] NATL UNIV SINGAPORE,DEPT CHEM,KENT RIDGE,SINGAPORE 0511,SINGAPORE

[2] NATL UNIV SINGAPORE,DEPT ZOOL,SINGAPORE 0511,SINGAPORE

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 13期

关键词：

D O I：

10.1080/01483919108049332

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The reversed-phase high-performance liquid chromatographic separation of several steroids is described in this paper. The optimization of the solvent composition was achieved by making use of the overlapping resolution mapping scheme. The optimum solvent composition was arrived at from only seven chromatographic experiments. Satisfactory separation was possible for all but one pair of the steroids considered.

引用

页码：2445 / 2455

页数：11

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