PURIFICATION AND CHARACTERIZATION OF RECOMBINANT EQUINE INFECTIOUS-ANEMIA VIRUS REVERSE-TRANSCRIPTASE

被引：17

作者：

LEGRICE, SFJ

PANIN, M

KALAYJIAN, RC

RICHTER, NJ

KEITH, G

DARLIX, JL

PAYNE, SL

机构：

[1] CASE WESTERN RESERVE UNIV, SCH MED, DEPT MOLEC BIOL & MICROBIOL, CLEVELAND, OH 44106 USA

[2] ECOLE NORMALE SUPER LYON, BIOL MOLEC & CELLULAIRE LAB, F-69364 Lyon, FRANCE

[3] INST BIOL MOLEC & CELLULAIRE, F-67084 STRASBOURG, FRANCE

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 12期

关键词：

D O I：

10.1128/JVI.65.12.7004-7007.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 1.67-kb segment of the equine infectious anemia virus pol gene, encoding a 66-kDa reverse transcriptase (RT), was cloned and expressed in Escherichia coli. Recombinant RT, purified by a combination of metal chelate affinity chromatography and ion-exchange chromatography, displays both RNA-dependent DNA polymerase and RNase H activity. The affinity of purified RT for its replication primer, tRNA3Lys was equivalent to that observed for human immunodeficiency virus RT. Our data suggest that an additional domain between RT-RNase H and integrase on the equine infectious anemia virus pol open reading frame is not an integral component of the RT polypeptide.

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页码：7004 / 7007

页数：4

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